The FOx technology uses fiber-optic-based surface plasmon resonance (FO-SPR) to directly measure an analyte of interest from blood, resuspended dried blood spots and other crude matrices. Providing accurate results from a range of sample inputs demonstrates the robustness and versatility of the FO-SPR technology.
- Serum
- Plasma
- Whole blood
- Dried blood spots
- Cell supernatant
- Cell lysate
Case study: Dry blood spot sampling
Dry blood spots are an ideal method for patients to provide blood samples from home, with minimal transportation requirements. However, blood cell bursting, and the altered structure of analytes caused by the drying process, can influence the detection efficiency.
In tests with infliximab (IFX), a monoclonal antibody used for the treatment of inflammatory bowel disease, the FO-SPR bioassay demonstrated that:
- The blood drying process did not affect analyte extraction or interfere with the bioassay antibodies.
- IFX was uniformly distributed across the blood spot on the paper.
- Non-specific binding due to blood cell bursting did not interfere with the FO-SPR bioassay.
Case study: Detecting a tumor protein in serum using gold nanoparticle sandwich amplification
The Pharmacology Department at the University of Cambridge are investigating a potential biomarker to detect triple negative breast cancer tumors in blood serum. Traditional technologies (ELISA, mass spectroscopy and conventional SPR) had proven unsuitable for their purposes, so they looked to the WHITE FOxTM for an alternative method.
FO-SPR with gold nanoparticle signal amplification matched or improved on the detection sensitivity of existing SPR technology. This allowed the development of a test suitable to retrospectively test patient samples for the candidate biomarker.
Relevant publications
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White paper: WP9 Isolation of neuron-specific extracellular vesicles from human plasma using FO-SPR
January 25, 2024
Measuring ADAMTS-13 activity to diagnose thrombotic thrombocytopenic purpura: a novel, fast fiber-optic surface plasmon resonance immunoassay
September 27, 2023
Detection of Breast Cancer-Specific Extracellular Vesicles with Fiber-Optic SPR Biosensor
February 15, 2023
Innovative FO-SPR Label-free Strategy for Detecting Anti-RBD Antibodies in COVID-19 Patient Serum and Whole Blood
January 27, 2022
White paper: WP3 Sensitive protein quantification in whole blood, dried blood spots, serum and plasma
June 29, 2021
White paper: WP2 sensitive protein quantification in serum – comparison of FO-SPR and ELISA
April 23, 2021
WHITE FOxTM technical specifications
April 8, 2021
FO-SPR biosensor calibrated with recombinant extracellular vesicles enables specific and sensitive detection directly in complex matrices
February 23, 2021
Immunoassay for detection of infliximab in whole blood using a fiber-optic surface plasmon resonance biosensor
February 22, 2017
Competitive inhibition assay for the detection of progesterone in dairy milk using a fiber optic SPR biosensor
January 15, 2017
Fiber optic-SPR platform for fast and sensitive infliximab detection in serum of inflammatory bowel disease patients
May 15, 2016
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For Research Use Only. Not for use in diagnostic procedures.